Composite

Part:BBa_K5403013:Design

Designed by: Lizette van der Ziel   Group: iGEM24_TU-Eindhoven   (2024-10-01)


Fusion protein for OmpA-based membrane anchoring of GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 412
    Illegal AgeI site found at 566
    Illegal AgeI site found at 591
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 528
    Illegal SapI.rc site found at 827


Design Notes

A flexible linker with the sequence GSGSGSGSAS was used to fuse the membrane anchoring domain and GFP. The flexibility prevents the individual domains from misfolding as a result of being fused to one another. Several modelling tools were used to aid in the design (DeepTMHMM, Alphafold).

Source

E. coli (OmpA) and Aequorea victoria (GFP).

References